TABLE 2.

Primers and PCR conditions

Species/genea	Primer nameb	Primer sequence (5′–3′)c	Annealing temperature/ cyclesd	Sourcee
Woolly rhino/12S rDNA	21pheF	AAAGCAAGGCATTGAAAATGCCTAGATGA	55°/×40	Tougard et al. (2001)
	684-12Sr	GGCGGTATATAGGCTGAATT
Woolly rhino/12S rDNA	650-12sF	CCGATAAACCCCGATAAACC	56°/×40	This study
	1085valR	TGAAATCTCCTGGGTGTAAGC
Woolly rhino/Cytochrome b	14228glu-2F	ACCAATGACATGAAAAATCATCGTT	52°/×40	This study
	14596cytbR	TTTCAGGTTTCTAGGAAGGTGT
Woolly rhino/Cytochrome b	14279cytbF	ATGACTAACATCCGCAAATCCC	66°/×40	This study
	15107cytbR	GGGATGGATCGTAGGATTGCGTA
Woolly rhino/Cytochrome b	14912cytbF	CCAACATAGACAAAATCCC	52°/×40	This study
	15446thrR	CCTTTTCTGGTTTACAAGACC
Woolly rhino/Real time PCR 1	WR_QPCR_415F	ACGTCCTACCATGAGGCCAA	55°/×40	This study
	WR_QPCR_484R	GGGATAGCTGAGAGAAGGTTTGTG
Woolly rhino/ Real time PCR 2	WR_QPCR_411F	GGCTACGTCCTACCATGAGGC	55°/×40	This study
	WR_QPCR_476R	TGAGAGAAGGTTTGTGATGACTGTG
Woolly rhino/Numt-1	66WR-PseudoF	GTAAGCATATGGTAAGCAC	55°/×50	This study
405WR-PseudoR	GCTACACTTTGGTTTATCCAACTCC
Pig / Mt. control region	L15387	CTCCGCCATCAGCACCCAAAG	56°/×40	Larson et al. (2005)
	H648n	GCTYATATGCATGGGGACT
Pig / CD45	PigEx9f	GAGAAATACATGGATATCCCTG	56°/×40	This study
PigEx9r	CTGGAGGTGTCTCTAAGAGG
Lion / ATP8 and num.	ATP8_1F	GCCACAGTTAGATACATC	56°/×40	I. Barnes, personal communication
	ATP8_3R	GAGGTGAATAGATTTTCGTTC
Moa / Mt. control region	185fm-CR	GTACATTCCCTGCATTGGCTC	55–60°/×40	Bunce et al. (2003)
	294rm-CR	GCGAGATTTGAACAGTACG
Moa / Mt. control region	262fm-CR	GCGAAGACTGACTAGAAGC	55–60°/×40	Bunce et al. (2003)
	441rm-CR	CGCATACCGGGTCTGTTTATGC
Moa / KW1 gene (sex linked)	Kw1-185f	GGCYRYTGCCTCAGAAATTACAG	52–55°/×40–50	Bunce et al. (2003)
	Kw1-260r	ATGCTATACTGCTTTAACAGA
Moa / ADH gene	Adh-230f	GAGGAATTAGCTYRTTAGCTGTC	52–55°/×40–50	Bunce et al. (2003)
	Adh-290r	GGTTAACACTTTGCCAGTGG

^aSpecimen name and sequence region amplified.

^bPrimer name.

^cPrimer sequence.

^dAnnealing temperature/ no. of PCR cycles.

^ePrimer source.