Figure 2.

Temperature sensitivity of zds1-disrupted and zds1-overexpressing cells. (A) Wild-type and zds1-disruptant strains or those harboring the indicated plasmids were grown for 14 days at 16° on the indicated plates and growth was observed. (B) Wild type S. pombe cells (SP66) that overexpress zds1 due to transformation with pREP1–zds1 were spotted onto PMA plates in the presence or absence of thiamine, absence of which induces the expression of zds1 from the plasmids. The cells were incubated at 25°, 30°, or 36.5° for 5 days and growth was observed.