Figure 8.
Susceptibility of the zds1Δ strain to zymolyase. Cells were cultured in PMA medium at 30° to a density of 1 × 107 cells/ml and then harvested and resuspended in TE (pH 8.0). Cells were incubated with 0.1 mg/ml zymolyase 20T at 30° for the indicated time. SP66/pREP41 (open square), MY6010/pREP41 (open circle), and MY6010/pREP41–zds1 (open triangle) were grown in the presence of thiamine. SP66/pREP41 (solid square), MY6010/pREP41 (solid circle), and MY6010/pREP41-zds1 (solid triangle) were grown without thiamine.