TABLE 2.
Primer | Sequence |
---|---|
SAMH601F | 5′-ATGTCGAC (SalI) AGAATTC (EcoRI) ATGTCTAGTTCTTCAGTT-3′ |
SAMH601R | 5′-ATGTCGAC (SalI) GGATCC (BamHI) TTTAGCATTCTTTGAAAC-3′ |
zds1-GFP5 | 5′-ATGAATTC (EcoRI) GCGGCCGC (NotI) ATGTCTAGTTCTTCAGTT-3′ |
zds1-GFP3 | 5′-ATGAATTC (EcoRI) GCGGCCGC (NotI) TTTTAGCATTCTTTGAAAC-3′ |
PH1–179 | 5′-AATTTAAAGCGGCCGC (NotI) CGGTAGATGCATCTTGTTC-3′ |
PH1–226 | 5′-TTTTAAAAGCGGCCGC (NotI)CTAGGGAACGAATGTGCTC-3′ |
PH1–567 | 5′-ATATGCATGCGGCCGC (NotI) CATAACCCTCAGTTTTATT-3′ |
PH1–669 | 5′-ATAAATTTGCGGCCGC (NotI)CACCTTTGTTAGCAGTGGA-3′ |
PH1–817 | 5′-GGCATATTGCGGCCGC (NotI)CACTCCAGTAATAATCCTT-3′ |
PH194–938 | 5′-AATCGAAAGCGGCCGC (NotI) ATGACTGGTTGGAAATCGTTT-3′ |
PH239–938 | 5′-TTTTAAAAGCGGCCGC (NotI) ATGGCTGACGATGCTGTAATT-3′ |
PH573–938 | 5′-ATGCAGCTGCGGCCGC (NotI) ATGCGTGAGGAAAAGAATTAT-3′ |
PH682–938 | 5′-TAAATTTTGCGGCCGC (NotI)ATGAAGAAAACCAAGAGGTCC-3′ |
Restriction enzyme sites are underlined.