TABLE 3.
zds1 expression reverses the sporulation deficiency of the ras1− strain but has no effect on byr2−, byr1−, and spk1− sporulation
| Sporulation rate (%)
|
|||
|---|---|---|---|
| Host | Plasmid | +Thiamine | −Thiamine |
| SPRN1DA (ras1−/ras1−) | pREP1 | 0.1 | 0.3 |
| pREP1-zds1 | 0.7 | 11.2 | |
| pREP41 | 0.1 | 0.7 | |
| pREP41-zds1 | 0.2 | 2.9 | |
| SPSUD (byr2−/byr2−) | pREP1 | 0 | 0 |
| pREP1-zds1 | 0 | 0 | |
| SPBUD (byr1−/byr1−) | pREP1 | 0 | 0 |
| pREP1-zds1 | 0 | 0 | |
| SPKUD (spk1−/spk1−) | pREP1 | 0 | 0 |
| pREP1-zds1 | 0 | 0 | |
The strains were streaked onto SPA plate and grown at 30° for 2 days. About 1000 cells were counted under the microscope.