TABLE 4.

Sporulation efficiency of the truncated zds1 gene

Plasmid	Sporulation rate (%)
pSLF172L GFP(S65A)	0.3
pSLF172L Zds1–GFP	5.8
pSLF172L Zds1(1–179)–GFP	0.7
pSLF172L Zds1(1–226)–GFP	0.4
pSLF172L Zds1(1–567)–GFP	0.3
pSLF172L Zds1(1–669)–GFP	0.4
pSLF172L Zds1(1–817)–GFP	0.3
pSLF172L Zds1(194–938)–GFP	21.9
pSLF172L Zds1(239–938)–GFP	11.8
pSLF172L Zds1(573–938)–GFP	17.9
pSLF172L Zds1(682–938)–GFP	7.0
pSLF172L Zds1(194–817)–GFP	0.7
pSLF172L Zds1(239–567)–GFP	0.7
pSLF172L Zds1(682–817)–GFP	0.4

SPRN1DA (ras1⁻/ras1⁻) was transformed with the above plasmids, streaked onto SPA plate, and grown at 30° for 2 days. About 1000 cells were counted under the microscope.