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Genetic interactions of MOT1 with NHP6. (A) nhp6ab mot1(R1243I) is lethal at 30°. Strains DY150 (wild type), DY7139 (nhp6ab), DY7463 [mot1(R1243I)], and DY7847 [nhp6ab mot1(R1243I)] were plated on YEPD medium for 4 days at 25° or for 2 days at 30°. (B) Strain DY7847 [nhp6ab mot1(R1243I)] was transformed with the indicated multicopy plasmids at 25°, and dilutions were plated on the indicated selective medium for 5 days at the indicated temperature.
