Skip to main content
. 2006 Feb;172(2):837–849. doi: 10.1534/genetics.105.050245

Figure 5.

Figure 5.

Suppression of mot1 spt15 and ccr4 spt15 synthetic lethality. (A) The mot1(R1243I) spt15(G174E) [TBP(G174E)] synthetic lethality is suppressed by TFIIA or Nhp6 overexpression. Strain DY9383 [mot1(R1243I) spt15Δ with a YCp–URA3–SPT15(wild-type) plasmid] was transformed with the YCp–TRP1–TBP(G174E) plasmid and the indicated LYS2 multicopy plasmids and grown for 3 days on complete medium at 25° or on 5-FOA medium at 34°. (B) The ccr4 spt15 synthetic lethalities are suppressed by TFIIA overexpression. Strain DY9384 [ccr4 spt15Δ with a YCp–URA3–SPT15(wild-type) plasmid] was transformed with either the YCp–TRP1–TBP(G147W) or the YCp–TRP1–TBP(L172P) plasmid and the indicated LYS2 multicopy plasmids and grown at 35° on complete medium for 2 days or on 5-FOA medium for 3 days. (C) Synthetic growth defect in the ccr4 mot1 double mutant. Strains DY150 (wild type), DY7462 [mot1(R1243I)], DY7176 (ccr4), and DY9470 [ccr4 mot1(R1243I)] were grown on complete medium at 30° for 2 days. (D) No additive effect in the not4 mot1 double mutant. Strains DY150 (wild type), DY7462 [mot1(R1243I)], DY8617 (not4), and DY9545 [not4 mot1(R1243I)] were grown on complete medium at 30° for 4 days. (E) mot1 suppresses the not5 growth defect. Strains DY150 (wild type), DY7462 [mot1(R1243I)], DY8627 (not5), and DY9582 [not5 mot1(R1243I)] were grown on complete medium at 30° for 3 days.