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. 2006 Feb;172(2):837–849. doi: 10.1534/genetics.105.050245

Figure 7.

Figure 7.

HO expression is reduced in mutant strains. (A) RNA was prepared from strains DY150 (wild type) and DY7462 [mot1(R1243I)] grown at either 25° or 30° and used for S1 nuclease protection assays to measure HO, CLN2, and CMD1 (internal control) RNA levels. (B) RNA was prepared from strains DY150 (wild type), DY7463 [mot1(R1243I)], DY5265 (gcn5), DY7841 [gcn5 mot1(R1243I)], DY7139 (nhp6ab), and DY7847 [nhp6ab mot1(R1243I)] grown at 25° and used for S1 nuclease protection assays to measure HO and tRNA–Trp (internal control) RNA levels.