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. 2006 Feb;172(2):963–974. doi: 10.1534/genetics.105.045286

Figure 3.

Figure 3.

Localization of DCC and MLE looks different in the regions of prominent puffs developed in the distal part of the X chromosome. (A–D) Oregon-R (wild type) male. (E) w; msl3 [w+; H83M2-61]/TM6 transgenic female. (F) Oregon-R female. (A and C) Anti-MSL2 detection. (B, D, E, and F) Anti-MLE detection. In the distal part of the X chromosome, temporal changes of gene expression are manifested by dynamic puffing in the regions 3C8-12 (from PS1 to PS4), 2B3-5 (from PS2 to PS10), and 2C1-2 (from PS5 to PS8). DCC associates differentially with the prominent 2B puff and is absent from the developed 2C (A) and 3C (C) puffs, whereas MLE binds diffusely to all these puffs (B and D). MLE binding appears intensive in the 3C puff in transgenic females, which produce less DCC than the wild-type male does (E). MLE predominantly binds puffs on female X chromosomes (F). Bar, 5 μm.