TABLE 2.
magellan-4 insertions identify 31 new genes required for S motility
| Gene | Name | Putative function |
|---|---|---|
| Central metabolic functions | ||
| MXAN0358 (1) | sgmA; ileS | Isoleucyl tRNA synthetase; downstream of fimT, pilW |
| MXAN3759 (1) | sgmM; pccB2 | Propionyl-CoA carboxylase |
| MXAN3797 (1) | sgmN | Short-chain acyl-CoA dehydrogenase |
| Cytoplasmic and membrane proteins of unknown function | ||
| MXAN0440 (1) | sgmB | Inner membrane protein; RND exporter |
| MXAN1106 (2) | sgmC | DnaJ/TPR repeat domain protein |
| MXAN1641 (1)a | sgmD | Hypothetical protein |
| MXAN1795 (1) | sgmE | Lipoprotein |
| MXAN2526 (1) | sgmH | Hypothetical protein |
| MXAN4150 (1) | sgmO | Conserved hypothetical protein; downstream of frzS |
| MXAN4620 (1) | sgmR | Hypothetical protein; downstream of rfbC; methyltransferase |
| MXAN4639 (1) | sgmS | TPR repeat protein; PilF domain |
| MXAN5766 (1) | sgmX | TPR repeat protein; PilF domain |
| MXAN5770 (1) | sgmY | Conserved hypothetical protein |
| MXAN6125 (1) | sgnA | Hypothetical protein; helicase? |
| MXAN6518 (1) | sgnB | Conserved hypothetical protein; ABC transporter |
| MXAN7360 (1) | sgnE | Lipoprotein |
| MXAN7442 (2) | sgnF | Membrane protein |
| Regulators of transcription | ||
| MXAN2128 (1) | sgmF | LysR family activator |
| MXAN4640 (1) | sgmT | Histidine kinase/response regulator |
| MXAN5592 (1) | sgmW | Response regulator |
| MXAN6627 (1) | sgnC | Response regulator |
| Polysaccharide metabolism | ||
| MXAN2203 (1) | sgmG | Asp box motif protein; glycosyl hydrolase? |
| MXAN2561 (1) | sgmI | Fibronectin type III domain protein; hydrolase? |
| MXAN2921 (1) | sgmJ; wbaZ | Glycosyl transferase group I domain |
| MXAN2922 (2) | sgmK | LPS biosynthesis; WcaJ C-terminal domain |
| MXAN3506 (4) | sgmL | Polysaccharide biosynthesis |
| MXAN4613 (1) | sgmP; rfbB2 | dTDP-glucose 4,6-dehydrogenase |
| MXAN4616 (3) | sgmQ | Glycosyl transferase group I and II domains |
| MXAN4707 (1) | sgmU; rfaF | Heptosyl transferase 9 domain |
| MXAN5333 (1) | sgmV; rfaG | Glycosyl transferase group I domains (2) |
| MXAN5831 (1) | sgmZ; glgP | Glycogen phosphorylase |
| MXAN6908 (3) | sgnD; pgi | Glucose-6-phosphate isomerase |
Genes are divided into four functional categories as shown and are numbered in accord with the M. xanthus genome sequence available at http://cmr.tigr.org/tigr-scripts/CMR/GenomePage.cgi?org=gmx. After each gene number, in parentheses is shown the number of magellan-4 insertions characterized in each gene; new genes involved in S motility were designated sgmA through sgnF; alternative suggestions for gene names are also listed. The predicted function of each gene is listed.
a
We have included MXAN1641 as an S gene although its direct role in motility has yet to be determined (see results).