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. 2006 Mar;172(3):1577–1593. doi: 10.1534/genetics.105.047100

Figure 3.

Figure 3.

Sb-sbd constructs for transgenic lines. Also shown is the location of the epitope for the stubblin stem (stem Ab) antiserum. The wild-type Sb-sbd (Sb+) construct includes the full-length Sb-sbd cDNA. The protease-disabled construct (S737A) substitutes an alanine for the catalytic serine at aa 737. The Δ-protease construct deletes the wild-type cDNA coding for the protein sequence C-terminal of aa 517 and is therefore missing the entire protease domain. The CD–TM–knot construct deletes the wild-type cDNA coding for the protein sequence C-terminal to residue 257 and thus retains the cytoplasmic domain and extracellular sequence through the disulfide knot (knot), but deletes most of the S/T-rich stem and all of the protease domain. CD–TM also deletes the disulfide knot, and CD expresses only the intracellular domain. CD–TM and CD constructs were gifts from C. Bayer.