TABLE 2.
Bristle phenotype
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Genotype | Treatment | n | % sbd | % Sb | % int | % wt |
hs-Sb+/hs-Sb+;sbd2/sbd2 | hs1 | 87 | 0 | 0 | 0 | 100 |
No hs | 53 | 100 | 0 | 0 | 0 | |
hs-Sb+/hs-Sb+;sbd201/Df(3R)sbd105 | hs1 | 50 | 0 | 0 | 10 | 90 |
No hs | 64 | 100 | 0 | 0 | 0 | |
S737A/S737A; sbd2/sbd2 | hs1 | 65 | 100 | 0 | 0 | 0 |
No hs | 86 | 100 | 0 | 0 | 0 | |
hs-Sb+/hs-Sb+; Sbspike/+ | hs1 | 63 | 0 | 38 | 43 | 19 |
No hs | 72 | 0 | 100 | 0 | 0 | |
hs-Sb+/hs-Sb+; Sb63b/+ | hs1 | 55 | 0 | 96 | 4 | 0 |
hs2 | 58 | 0 | 100 | 0 | 0 | |
No hs | 84 | 0 | 100 | 0 | 0 |
Homozygote sbd2/sbd2, deficiency trans-heterozygote sbd201/Df(3R)sbd105 or heterozygote Sb63b/+, and Sbspike/+ stocks carrying two copies of the wild-type Sb-sbd transgene (hs-Sb+/hs-Sb+), and sbd2/sbd2 carrying two copies of the protease-disabled S737A transgene were collected as white prepupae, aged at 25° until 27.5 hr AP, and then either heat shocked at 37° for 1 hr (hs1) or maintained at 25° (no hs). Another set of Sb63b pupae carrying the wild-type transgene was heat shocked at 24, 26, and 28 hr AP (hs2). After heat shock, pupae were returned to 25° until eclosion. Adults were scored for wt, sbd, Sb, or intermediate (int) phenotype bristles (n: number of adults; % wt, sbd, Sb, or int: percentage of each phenotype observed).