. 2006 Mar;172(3):1577–1593. doi: 10.1534/genetics.105.047100

TABLE 2.

Rescue of bristle phenotype with heat-shock induction of wild-type Sb-sbd transgene

		Bristle phenotype
Genotype	Treatment	n	% sbd	% Sb	% int	% wt
hs-Sb⁺/hs-Sb⁺;sbd²/sbd²	hs1	87	0	0	0	100
No hs	53	100	0	0	0
hs-Sb⁺/hs-Sb⁺;sbd²⁰¹/Df(3R)sbd¹⁰⁵	hs1	50	0	0	10	90
No hs	64	100	0	0	0
S737A/S737A; sbd²/sbd²	hs1	65	100	0	0	0
No hs	86	100	0	0	0
hs-Sb⁺/hs-Sb⁺; Sb^spike/+	hs1	63	0	38	43	19
No hs	72	0	100	0	0
hs-Sb⁺/hs-Sb⁺; Sb^63b/+	hs1	55	0	96	4	0
hs2	58	0	100	0	0
	No hs	84	0	100	0	0

Homozygote sbd²/sbd², deficiency trans-heterozygote sbd²⁰¹/Df(3R)sbd¹⁰⁵ or heterozygote Sb^63b/+, and Sb^spike/+ stocks carrying two copies of the wild-type Sb-sbd transgene (hs-Sb⁺/hs-Sb⁺), and sbd²/sbd² carrying two copies of the protease-disabled S737A transgene were collected as white prepupae, aged at 25° until 27.5 hr AP, and then either heat shocked at 37° for 1 hr (hs1) or maintained at 25° (no hs). Another set of Sb^63b pupae carrying the wild-type transgene was heat shocked at 24, 26, and 28 hr AP (hs2). After heat shock, pupae were returned to 25° until eclosion. Adults were scored for wt, sbd, Sb, or intermediate (int) phenotype bristles (n: number of adults; % wt, sbd, Sb, or int: percentage of each phenotype observed).