. 2006 Mar;172(3):1577–1593. doi: 10.1534/genetics.105.047100

TABLE 3.

Rescue of mlf legs by heat-shock induction of the wild-type Sb-sbd transgene

		Leg phenotypes
Genotype	Treatment	n	% mlf	% wt
hs-Sb⁺/hs-Sb⁺; sbd²⁰¹/sbd²⁰¹	+hs	63	68	32
−hs	85	89	11
hs-Sb⁺/hs-Sb⁺; sbd²⁰¹/Df(3R)sbd¹⁰⁵	+hs	72	67	33
−hs	58	93	7
hs-Sb⁺/+; sbd²⁰¹/Df(3R)sbd¹⁰⁵	+hs	117	54	46
−hs	118	91	9
+/+; sbd²⁰¹/sbd²⁰¹	+hs	52	94	6
−hs	88	96	4
S737A/+; sbd²⁰¹/Df(3R)sbd¹⁰⁵	+hs	121	100	0
	−hs	122	93	7

White prepupae from sbd²⁰¹/sbd²⁰¹ or sbd²⁰¹/Df (3R) sbd¹⁰⁵ stocks carrying zero (+/+), one (hs-Sb⁺/+), or two (hs-Sb⁺/hs-Sb⁺) copies of the wild-type Sb-sbd transgene or one copy of the S737A protease-disabled transgene were either heat shocked for 1 hr at 37° immediately after collection at 0 hr AP (+hs) or maintained at 25° (−hs). Adults were scored for the mlf leg phenotype. The number scored (n) and percentage with mlf (% mlf) and wt (% wt) phenotypes are shown.