TABLE 3.
Leg phenotypes
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Genotype | Treatment | n | % mlf | % wt |
hs-Sb+/hs-Sb+; sbd201/sbd201 | +hs | 63 | 68 | 32 |
−hs | 85 | 89 | 11 | |
hs-Sb+/hs-Sb+; sbd201/Df(3R)sbd105 | +hs | 72 | 67 | 33 |
−hs | 58 | 93 | 7 | |
hs-Sb+/+; sbd201/Df(3R)sbd105 | +hs | 117 | 54 | 46 |
−hs | 118 | 91 | 9 | |
+/+; sbd201/sbd201 | +hs | 52 | 94 | 6 |
−hs | 88 | 96 | 4 | |
S737A/+; sbd201/Df(3R)sbd105 | +hs | 121 | 100 | 0 |
−hs | 122 | 93 | 7 |
White prepupae from sbd201/sbd201 or sbd201/Df (3R) sbd105 stocks carrying zero (+/+), one (hs-Sb+/+), or two (hs-Sb+/hs-Sb+) copies of the wild-type Sb-sbd transgene or one copy of the S737A protease-disabled transgene were either heat shocked for 1 hr at 37° immediately after collection at 0 hr AP (+hs) or maintained at 25° (−hs). Adults were scored for the mlf leg phenotype. The number scored (n) and percentage with mlf (% mlf) and wt (% wt) phenotypes are shown.