TABLE 4.

Overexpression of wild-type Sb-sbd causes defects in leg morphogenesis

Genotype	Treatment	n	% wt
hs-Sb+/hs-Sb+; +/+	0 hr AP hs	118	54
3 hr AP hs	58	50
30 and 33 hr AP hs	55	0
No hs	120	100
No transgene	0 hr AP hs	88	96
3 hr AP hs	56	93
30 and 33 hr AP hs	70	100
	No hs	70	100

White prepupae from wild-type flies carrying two copies of the wild-type Sb-sbd transgene (hs-Sb⁺) or no transgene were heat shocked immediately (0 hr AP hs) or aged at 25° until 3 hr AP and then heat shocked (3 hr AP). A third group was heat shocked at 30 hr and again at 33 hr AP (30 and 33 hr AP). All heat shocks were at 37° for 1 hr. Adults (n) were scored for leg phenotype. The percentage of individuals with all wild-type legs (no legs malformed) is shown (% wt). See text for a description of differences between malformed leg phenotypes resulting from early and late heat shock.