TABLE 4.
Genotype | Treatment | n | % wt |
---|---|---|---|
hs-Sb+/hs-Sb+; +/+ | 0 hr AP hs | 118 | 54 |
3 hr AP hs | 58 | 50 | |
30 and 33 hr AP hs | 55 | 0 | |
No hs | 120 | 100 | |
No transgene | 0 hr AP hs | 88 | 96 |
3 hr AP hs | 56 | 93 | |
30 and 33 hr AP hs | 70 | 100 | |
No hs | 70 | 100 |
White prepupae from wild-type flies carrying two copies of the wild-type Sb-sbd transgene (hs-Sb+) or no transgene were heat shocked immediately (0 hr AP hs) or aged at 25° until 3 hr AP and then heat shocked (3 hr AP). A third group was heat shocked at 30 hr and again at 33 hr AP (30 and 33 hr AP). All heat shocks were at 37° for 1 hr. Adults (n) were scored for leg phenotype. The percentage of individuals with all wild-type legs (no legs malformed) is shown (% wt). See text for a description of differences between malformed leg phenotypes resulting from early and late heat shock.