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. 2006 Mar;172(3):1545–1555. doi: 10.1534/genetics.105.051490

Figure 2.

Figure 2.

Triheterokaryon tests with the pin-c mutants. (A) A XK5 (het-c2 pin-c2m) + IK09-2 (het-c2 pin-c2) + IK09-51 (het-c1 pin-c1) triheterokaryon grown at 22° or 34° shows full complementation of HI at both temperatures. (B) By contrast, a XK108 (het-c2 pin-c2m) + CJ44 (Δhet-c2 Δpin-c2) + IK09-15 (het-c1 pin-c1) triheterokaryon grown at 22° or 34° shows an identical phenotype to a heterokaryon between XK108 (het-c2 pin-c2m) and IK09-15 (het-c1 pin-c1), i.e., attenuated heterokaryon incompatibility at 22°, but full compatibility at 34°. Plates were incubated at 22° for 5 days (left) or 34° for 3 days (right).