Amino acid sequence alignment of PIN-C1, PIN-C2, and PIN-C3. The three conserved regions of the HET domain identified by alignment of 78 HET domain proteins (PFAM06985); regions that define the HET domain (I, II, and III) are underlined. The amino acid substitution Leu410Pro in the pin-c2m allele in XK5 is indicated with lowercase “p.” Solid background indicates amino acid identity, shaded boxes indicate conserved aa substitutions, and dots indicate deletion events.