Figure 6.
HI phenotype of heterokaryons having het-c or pin-c allelic or het-c/pin-c nonallelic interactions at 22° (A, B, and D) or 34° (C). A compatible heterokaryon (RLM 57-30 + FGSC 4564) (▪) and incompatible heterokaryon (FGSC 4564 + C9-2) (▴) were used as controls. (A) A (IRO42 + IRP216) heterokaryon (○) with a het-c allelic interaction only (het-c1 pin-c1m + het-c2 pin-c2m) was fully compatible. A IK11-20 + C7-47 heterokaryon (•) with a pin-c allelic interaction only (het-c1m pin-c1 + het-c2m pin-c2) was fully compatible. (B) A (IRO42 + C7-47) heterokaryon (•) with a het-c1/pin-c2 nonallelic interaction (het-c1 pin-c1m + het-c2m pin-c2) grew at ∼5 cm/day. A (IK11-20 + IRP228) heterokaryon (○) with a het-c2/pin-c1 nonallelic interaction (het-c1m pin-c1 + het-c2 pin-c2m) grew at ∼5.5 cm/day. (C) A (IRO42 + C7-47) heterokaryon (•) with a het-c1/pin-c2 nonallelic interaction grew at ∼5 cm/day. The (IK11-20 + IRP228) heterokaryon (○) with a het-c2/pin-c1 nonallelic interaction (het-c1m pin-c1 + het-c2 pin-c2m) was fully compatible (∼11.5 cm/day). (D) A (FGSC 4564 + C7-47) heterokaryon (•) containing het-c1/pin-c2 and pin-c1/pin-c2 interactions was identical in phenotype to a heterokaryon containing only a het-c1/pin-c2 interaction (IRO42 + C7-47) (B). A (IK11-20 + Xa-2) heterokaryon (○) with het-c2/pin-c1 and pin-c1/pin-c2 interactions was identical in phenotype to (IK11-20 + IRP228) (B). Predicted het-c or pin-c allelic or het-c/pin-c nonallelic interactions are shown in the insets as solid and open arrows, which correspond to solid or open circles in A–D.