TABLE 3.
Ptr2p-GFP expression and localization in 103 identified strains with decreased or increased dipeptide utilization
Phenotypic category | Increased dipeptide utilization (46 strains) | Decreased dipeptide utilization (57 strains) |
---|---|---|
Strains with enhanced Ptr2p-GFP expression | arp5, arp8, cup9, csm1, dbr1, eaf3, hal9, ies6, kem1, mrps9, pat1, sfp1, srb8, ssd1, ssn2, ssn3, ssn8, tom72, ubr2, yor322c, ynl123w, yfr044c, ylr114c | asm4, ydr015c, ydr290w |
Strains with altered Ptr2p-GFP localization | bni1, def1, eaf7, lst4, npr1, sac6, snf7, srn2, tpm1, vam10, vps8, vps36, ypl073c, ynl295w | bem4, bud32, csf1, etr1, gcv2, gcv3, hfm1, ilm1, isa1, isa2, mck1, mre11, npl3, shp1, reg1, rmd12, rps9b, tsa1, vbm1, ydr433w, yjl046w, ydr157w, yjl175w |
Strains with no apparent effect on Ptr2p-GFP localization or expression | cik1, kti12, mlh1, mrp17, pho2, rim101, tif3, ybt1, ydr417c | bud28, bye1, elf1, hfa1, ira2, lat1, lpd1, mct1, oar1, pdb1, pdx1, prd1, rad23, spo21, ypl098c |
Strains with decreased Ptr2p-GFP expression | No mutant strains were found in this category. | dal81, hof1, ipk1, nfu1, pho85, ptr1, rpl21a, rpn4, shm2, stp2, taf14, thp1, uba3, ubp14, ubc2, ypr174c |
Ptr2p-GFP expression and localization in the underlined strains are shown in Figures 3 and 4. The underlined strains were the representatives chosen to highlight in the results section. Ptr2p-GFP expression and localization of all the listed strains are shown in supplemental Figures S1 and S3 at http://www.genetics.org/supplemental/. The strains in boldface type (cup9, dal81, ptr1, stp2, and ubc2) contain the deleted genes previously identified as being involved in regulation of PTR2 transcription.