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. 2006 Mar;172(3):1521–1533. doi: 10.1534/genetics.105.047381

Figure 1.

Figure 1.

Construction of deletion strains. (A) Genomic pre1 region and construction of the Δpre1 gene replacement vector. Positions of primers used to amplify the disruption construct from plasmid pPRE1-KO and to verify the homologous recombination at the pre1 locus are indicated. (B) Genomic pre2 region and construction of the Δpre2 gene replacement vector. Positions of primers used to amplify the disruption construct from plasmid pPRE2-KO and to verify the homologous recombination at the pre2 locus are indicated. (C) The genomic ppg2 region and construction of the Δppg2 gene replacement vector. Positions of primers used to amplify the disruption construct from plasmid pPPG2-KO and to verify the homologous recombination at the ppg2 locus are indicated. PtrpC, A. nidulans trpC promoter.