Figure 2.

Transcript analysis of S. macrospora wild-type and mutant strains. (A) RT–PCR. pre1 (MrezS2, Mrez32)-, pre2 (PrezS3, PrezS4)-, and gpd (C-gpd, N-gpd)-specific oligonucleotide pairs were employed in an RT–PCR of S. macrospora total RNA. D, DNA template (S. macrospora wild type); -RT, RNA template (S. macrospora wild type), no reverse transcription prior to PCR. (B) Northern analysis. Twenty micrograms of total RNA, isolated at different time points during the life cycle of wild-type and single-mutant strains (at 3, 5, and 7 days) was loaded per lane. Northern blots were probed using a ppg1 and ppg2 gene-specific probe, respectively. As a control, the blots were striped and reprobed with a 5.8S rRNA-specific probe.