Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2006 May 2;34(8):2280–2293. doi: 10.1093/nar/gkl180

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2006. Published by Oxford University Press. All rights reserved

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

PMC Copyright notice

Expression and methylation analysis in hypomethylated Lo1E/Lo2 plants. (A) RNA gel-blot hybridization of the nptII RNA isolated from 5-aza-2-deoxy-cytidine (azaC)-treated (1–5) and non-treated (C) plants. The numbers below the lanes indicate relative signal intensities of the nptII-specific band. The signals were normalized by ethidium bromide fluorescence of the 25S rRNA gene (bottom panel). The signal in the control lane (C) was arbitrarily chosen as 100%. (B) DNA methylation in the endogenous HRS60 satellite analyzed by HpaII restriction enzyme. (C) DNA methylation in the 35S promoters analyzed by TaiI restriction enzyme (+) as described in Figure 5. DNAs were predigested with BglII to separate both loci. The ratio between methylated/non-methylated fragments are indicated below the lanes.