Figure 4.

Resolution across entire image. Full-frame image of an England Finder slide consisting of etched 1 mm squares on a thick glass substrate. The image was obtained using the Cy5 filter set. The excitation light produced fluorescence in the glass substrate, which back-illuminate the etched pattern on the upper surface. Enlarged images of two of these squares, one at the center and one at a corner of the image are shown, indicating that the focus is maintained across the entire field. The full-field encompasses approximately a 20 mm square, which given the 2K × 2K pixels, calibrates the pixelization as 10 µm/pixel.