Table 1.
List of oligonucleotides and probes
| Assayname | Primer 1 | Primer 2 | M probea | U probeb |
|---|---|---|---|---|
| APC-1 | 5′Biotin-GGAGAGAGAAGTAGTTGTGTAATT | ACTACACCAATACAACCACATATC | CT128-OOc-ACTCCGCAATA | CT156-OOc-ACTCCACAATA |
| APC-2 | 5′Biotin-GGAGAGAGAAGTAGTTGTGTAATT | ACTACACCAATACAACCACATATC | CT128-OOc-AATCCGCATCC | CT156-OOc-AATCCACATCC |
| APC-3 | 5′Biotin-GGAGAGAGAAGTAGTTGTGTAATT | ACTACACCAATACAACCACATATC | CT156-OOc-ATCGACGAACT | CT156-Oc-ATCAACAAACT |
| EYA4-1 | 5′Biotin-TAGGTTTTTGGAAGAGGTGA | CCCAAAAATCAAACAACAAC | CT128-OOc-ATTTCGTATCT | CT142-Oc-ATTTCATATCT |
| EYA4-2 | 5′Biotin-TTTTAGTAATGTGTTTAGAGAAGTTTTG | CCCAAAAATCAAACAACAAC | CT128-OOc-ACGACGT | CT128-Oc-ACAACAT |
| GSKB-1 | 5′Biotin-AGTGATAAAGGAAGGAAGGAA | CAACCCAAAACCCTATCAAC | CT128-OOc-TCAACGACTAC | CT128-Oc-TCAACAACTAC |
| GSKB-2 | 5′Biotin-AGTGATAAAGGAAGGAAGGAA | CAACCCAAAACCCTATCAAC | CT156-OOc-CGAAACCGACT | CT156-Oc-CAAACCAACT |
| GSKB-3 | 5′Biotin-AGTGATAAAGGAAGGAAGGAA | CAACCCAAAACCCTATCAAC | CT128-OOc-TCCTCGCTTCC | CT128-Oc-TCCTCACTTCC |
| hMLH1-1 | 5′Biotin-TTTTTAGGAGTGAAGGAGGTT | TAAAACCCTATACCTAATCT | CT156-OOc-CGTCTACGTCA | CT142-OOc-CATCTACATCA |
| hMLH1-2 | 5′Biotin-TTTTTAGGAGTGAAGGAGGTT | TAAAACCCTATACCTAATCT | CT142-OOc-CGCCACTACGA | CT128-Oc-CACCACTACAA |
| hMLH1-3 | 5′Biotin-TGAAGGGTGGGGTTGGAT | TTCAACCAATCACCTCAATACC | CT156-Oc-CTCACGTTCTT | CT128-OOc-CTCACATTCTT |
| MDR1-1 | 5′Biotin-GAAGATTTATGTGAATTTTGAAAG | AAACATTTCAACTTATATAAACAC | CT156-OOc-AACACGTCTTT | CT156-OOc-AACACATCTTT |
aM probe: PNA probe reverse complementary to methylated sequence
bU probe: PNA probe reverse complementary to unmethylated sequence.
cChemical modifications: O = O-linker ({2-[2-(Fmoc-amino)ethoxy]acetic acid; CT156 = N-Propyl-5-Trimethylammoniumpentylamid; CT142 = N-Propyl-4-Trimethylammonium-butyrylamid and CT128 = N-Propyl-3-Trimethylammonium-propylamid.