Table 2.
Characteristic values of evaluated assays
| Assay | PCR length(bp) | Number of CpGs in probe | C-content(%) in probe | Measurement rangea |
|---|---|---|---|---|
| APC-2 | 123 | 1 | 45 | 0.02 |
| APC-1 | 123 | 1 | 36 | 0.16 |
| EYA4-1 | 227 | 1 | 18 | 0.17 |
| GSK3β-3 | 66 | 1 | 55 | 0.18 |
| hMLH1-2 | 180 | 2 | 45 | 0.19 |
| hMLH1-3 | 77 | 1 | 36 | 0.26 |
| hMLH1-1 | 180 | 2 | 36 | 0.4 |
| GSK3β-1 | 66 | 1 | 36 | 0.54 |
| APC-3 | 123 | 2 | 27 | 0.57 |
| GSK3β-2 | 66 | 2 | 36 | 0.68 |
| MDR1-1 | 53 | 1 | 27 | 0.71 |
| EYA4-2 | 69 | 2 | 29 | 0.88 |
aDifference of measured methylation levels for methylated and unmethylated DNA = (CG/(CG+CA)meth − (CG/(CG+CA)unmeth) = Maximum range of measurable values. High values allow a higher resolution of methylation measurement.