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. 2006 May 2;34(8):2269–2279. doi: 10.1093/nar/gkl258

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© The Author 2006. Published by Oxford University Press. All rights reserved

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A plasmid-based, mobile D-loop is a substrate for BLM. (a) BLM-concentration dependent unwinding of pDL3 in a 15 min reaction. The enzyme concentration was varied (as indicated by the triangle above) between 0.7 and 5.6 nM in steps of 0.7. ‘0’ denotes a control sample in the absence of BLM, which was otherwise treated identically. (b) Unwinding of pDL3 in a time-course experiment. Samples were taken at the time points indicated above the lanes. The positions of the substrate and the free radiolabelled oligonucleotide are indicated on the left.