TABLE 4.
Interaction between ExoVII and SbcCD exonucleases affects conjugational recombination in recD mutants
| Recipient strain | Description | Relative cellular viability | Relative recombination frequency |
|---|---|---|---|
| DE100 | RecD− | 1.0a | 1.0b |
| DE1050 | RecD− ExoI− | 0.88 | 0.24 |
| DE1053 | RecD− SbcCD− | 1.05 | 0.81 |
| DE1052 | RecD− ExoVII− | 0.91 | 1.09 |
| DE1058 | RecD− ExoVII− SbcCD− | 0.83 | 0.88 |
| DE1056 | RecD− ExoI− SbcCD− | 0.81 | 0.23 |
| DE1054 | RecD− ExoI− ExoVII− | 0.76 | 0.93 |
| DE1060 | RecD− ExoI− ExoVII− SbcCD− | 0.55 | 0.41 |
a
Cellular viability of 1.0 corresponds to 1.2 × 108 colony-forming units ml−1 at an OD600 of 0.3.
b
Recombination frequency of 1.0 corresponds to 48 Pro+ transconjugants/100 IRB110 donor cells. Recombination efficiency is corrected for the viability of the recipients. All values are averages of two independent determinations.