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. 2006 Apr;172(4):2101–2112. doi: 10.1534/genetics.105.050955

Figure 4.

Figure 4.

Disruption of three major targets of ace2+ can rescue the growth defects of sid2-250 at lower restrictive temperature. (A) Serial dilution growth test was done with strains of mid2Δ (YDM1259), mid2Δ sid2-250 (YDM2055), eng1Δ (YDM1948), agn1Δ (YDM2801), sid2-250 (YDM429), eng1Δ sid2-250 (YDM2058), agn1Δ sid2-250 (YDM2802), eng1Δ agn1Δ (YDM2772), and eng1Δ agn1Δ sid2-250 (YDM2805). (B) Rescue of sid2-250 by ace2Δ can be reversed by overexpressing mid2+. Wild-type (YDM105), sid2-250 (YDM429), ace2Δ (YDM2028), and ace2Δ sid2-250 (YDM2027) cells transformed with either empty vector (pREP41) or the mid2+-expressing plasmid (pREP41–mid2+) were first grown in EMM plus thiamine medium and then washed, diluted, and dropped onto EMM plates without thiamine.