TABLE 3.
Summary of rescue of the sid2-250 mutant by some cell-separation mutations
| Mutants in | Strain | 25° | 30° | 33° | 36° |
|---|---|---|---|---|---|
| Ace2 targets | mid2Δ sid2-250 | ++ | ++ | ++ | − |
| eng1Δ sid2-250 | ++ | − | − | − | |
| agn1Δ sid2-250 | ++ | +/− | − | − | |
| eng1Δ agn1Δ sid2-250 | ++ | ++ | +/− | − | |
| Septins | spn3Δ sid2-250 | ++ | + | − | − |
| spn4Δ sid2-250 | ++ | ++ | ++ | − | |
| Exocyst complex | sec8-1 sid2-250 | ++ | +/− | − | − |
| Rho GTPase | rho3Δ sid2-250 | ++ | +/− | − | − |
| rho4Δ sid2-250 | ++ | + | − | − | |
| PP2A regulatory subunit | par1Δ sid2-250 | ++ | +/− | − | − |
| par2Δ sid2-250 | ++ | − | − | − | |
| par1Δ par2Δ sid2-250 | ++ | + | − | − | |
| Transcription factor | sep1 sid2-250 | ++ | − | − | − |
Growth was examined with a serial dilution drop test at different temperatures. ++, good growth; +, weak growth; +/−, weak growth with variations in growth in different clones; −, no growth.