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. 2000 Dec 19;98(1):176–181. doi: 10.1073/pnas.011511298

Figure 3.

Figure 3

Reproducibility and sensitivity of the pro MSS HTA. (A) The MSS HTA reflects the populations in the PCR product accurately and reproducibly at high template numbers. PCR products of two pro genes were mixed at known ratios; the mixtures were then diluted over a 100-fold range and annealed to the MSS HTA probe 6.1. The abundance of one of the products was determined by using the MSS HTA and compared with the known abundance. Error bars represent standard deviations from 5–9 experiments. (B) Amplification of pro gene mixtures. Mixtures of pro genes were amplified and quantified by using the MSS HTA. The smallest number of templates required to detect and quantify a 10% population reproducibly is 1,200. Error bars are the standard deviation from three experiments. The sequences used encoded the following changes from Hxb: (1) T12S, K43R, M46I, Q61L, L63P, V82A; (2) T12S, K43R, M46I, I54V, Q61H, L63P, V82F.