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. 2000 Dec 19;98(1):176–181. doi: 10.1073/pnas.011511298

Figure 6.

Figure 6

Development of an RT MSS HTA. (A) Sequence of the RT MSS HTA probe 2.1 (1) aligned to Hxb-2r wild-type sequence; (2) base changes involved in resistance (3) are shown above. (B) Mobility of the radioactively labeled probe when annealed to three PCR products containing resistance mutations in comparison with the mobility of wild type (wt). In addition, plasma RNA of Patient 1029 who started 3TC therapy at day 215 was subjected to an MSS HTA analysis shown on the same gel. hd, heteroduplex; dsP, double-stranded probe.