Figure 4.

Disruption of GAL11, SRB2, SRB10, or NUT1 has little or no affect on hsp82-ΔHSE1 expression. Wild-type (WT) and mutant strains were cultivated, split into NHS and HS aliquots, and assayed for β-gal activity as in Figure 3. (A) Noninduced hsp82-ΔHSE1/lacZ expression in WT (HS1001), gal11Δ (DAD3), srb2Δ (DAD2), and srb10Δ (RRG2) strains. (B) Heat-shock-induced expression of hsp82-ΔHSE1/lacZ (open bars) compared with nonshocked expression (solid bars) in the same strains. (C) hsp82-ΔHSE1/lacZ expression in WT and nut1Δ strains (HS1001 and JHD1, respectively) under NHS and HS conditions as indicated. (A–C) means ±SEM (n = 3).