Basal transcription of PHO5 is elevated in the context of certain ewe mutations. Northern analysis of wild-type (WT; HS1004), rgr1 (J1), rox3 (J34), srb7 (S2), nut2 (J15), and med7 (S5) strains cultivated in rich YPDA medium to early log phase in 30°. PHO5 transcript levels were normalized to those of ACT1. Depicted are means of two independent experiments ±SD.