TABLE 1.
Yeast strains
| Strain | Genotype | Source or reference |
|---|---|---|
| SLY101 | MATα ade- can1-100 cyh2r his3-11,15 leu2-3,112 trp1-1 ura3 | Lee and Gross (1993) |
| KEY102 | SLY101; hsp82-ΔHSE1 | Gross et al. (1993) |
| HDY1002 | KEY102; hsp82-ΔHSE1/HIS3∷URA3, TRP1+ | This study |
| HS3000 | HDY1002; hsp82-ΔHSE1/lacZ∷ LEU2 | This study |
| HS1001 | HS3000; hsp82-ΔHSE1-HIS3∷ura3Δ (KanMX excised by Cre recombinase) | This study |
| HS1002 | HS1001; MATa | This study |
| HS1003 | HS3000; leu2∷hsp82- ΔHSE1-lacZ∷leu2∷KanMX | This study |
| HS1004 | HS1003; MATa | This study |
| HS1005 | HS1001; sin4Δ∷KanMX | This study |
| J79 | HS1004; sin4-1001 | This study |
| J1 | HS1004; rgr1-1001 | This study |
| S5 | HS1004; med7-1001 | This study |
| J121 | HS1004; med7-1002 | This study |
| S2 | HS1004; srb7-1001 | This study |
| J20 | HS1004; srb7-1002 | This study |
| J15 | HS1004; nut2-1001 | This study |
| J84 | HS1004; nut2-1002 | This study |
| J34 | HS1004; rox3-1001 | This study |
| B20 | HS1004; rox3-1002 | This study |
| RRG2 | HS1001; srb10Δ∷KanMX | This study |
| DAD2 | HS1001; srb2Δ∷KanMX | This study |
| DAD3 | HS1001; gal11Δ∷KanMX | This study |
| JHD1 | HS1001; nut1Δ∷KanMX | This study |
| SBK2000 | J20; LEU2+ | This study |
| SBK2001 | HS1001; SRB7- KanMX | This study |
| SBK2002 | HS1001; met16Δ∷KanMX | This study |
| SBK2003 | HS1001; pfk27Δ∷KanMX | This study |
| SBK2004 | HS1001; ybl094CΔ∷KanMX | This study |
| SBK2005 | J121; LEU2+ | This study |
| SBK2006 | S5; LEU2+ | This study |
| SBK2007 | J15; LEU2+ | This study |
| SBK2008 | J84; LEU2+ | This study |
| SBK2009 | B20; LEU2+ | This study |
| SBK2010 | J34; LEU2+ | This study |
| DY150 | MATaade2-1 can1-100 his3-11,15 leu2-3,112 trp1-1 ura3-1 | D. J. Stillman |
| DY2694 | DY150; MATα rgr1-Δ2∷LEU2 | D. J. Stillman |
| SBK501 | MATα leu2∷hsp82-ΔHSE1-lacZ∷leu2∷KanMX trp1 ura3 rgr1-Δ2∷LEU2 | This study |
| S288C | MATahis3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | Invitrogen |
| HSF1-GFP | S288C; HSF1-GFP | Invitrogen |