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HSF occupancy levels
| Gene | Noninduced | Induced |
|---|---|---|
| HSP12 | 0.7 | 0.7 |
| HSP26 | 0.4 | 0.95 |
| HSP82 | 1.0 | 2.1 |
| HSC82 | 1.0 | 1.0 |
| SSA3 | 0.2 | 0.65 |
| SSA4 | 1.3 | 3.4 |
HSF ChIP signals at the indicated gene promoters were normalized to those of noninduced HSP82 and represent the means of at least two independent experiments. Cells were heat-shock induced at 39° for 15 min. For these assays, PHO5 served as a nonspecific ChIP control as previously described (Sekinger and Gross 2001).
