TABLE 4.

ADHII activities in adr7-1 (mot1) mutant strains

		Genotype
Line	Strain	MOT1	SNF1	ADR1a	ADH2	ADHII activity (R)b	AtRc
1	SSH35	WT	WT	220	WT	29	−
2	S54	adr7-1	WT	220	WT	195	+++
3	S54	adr7-1	WT	Δ	WT	50	−+
4	S54ΔS	adr7-1	Δ	220	WT	20	−
5	S54ΔS	adr7-1	Δ	Δ	WT	22	−
6	LK13	WT	WT	220	ADH2-10	240	+++
7	LV14	adr7-1	WT	220	ADH2-10	500	+++
8	LK13ΔS	WT	Δ	220	ADH2-10	18	−
9	LV14ΔS	adr7-1	Δ	220	ADH2-10	75	−+
10	LK13ΔS	WT	Δ	Δ	ADH2-10	15	−
11	LV14ΔS	adr7-1	Δ	Δ	ADH2-10	50	−+

Cells were grown in synthetic medium containing 5% glucose and crude cell extracts were assayed for ADH activity as described in materials and methods.

^aThe ADR1-220 allele was present on a CEN4-TRP1 plasmid (Karnitz et al. 1992). In strains denoted Δ in the ADR1 column the pRS314 vector was present.

^bThe ADH activity is the average of triplicate measurements that had a standard deviation of ∼10%.

^cGrowth in the presence of the respiratory inhibitor Antimycin A (At^R) is indicative of constitutive ADH2 expression (Karnitz et al. 1992). Growth was scored as good growth (+++), very poor growth (−+), or no growth (−) after replica plating patches from a plate containing synthetic Trp⁻ medium supplemented with 5% glucose.