Figure 2.

Cell-cycle distribution and nuclear morphology of smc5 cells. The percentages of cells that were unbudded, single budded, large budded, and anomalous were assayed microscopically at 22° (a) and at 36° (b). More than 300–700 cells per strain at both time points were counted while blind with respect to the genotype and temperature. (c) Asynchronous cultures of SMC5 (yGC141), smc5-31 (yGC231), and smc5-33 (yGC233) cells grown at 22° or at 36° for 6 hr were stained with propidium iodide and analyzed by FACS for DNA content. (d) DNA in cells from a were stained with 4′,6-diamidino-2-phenylindole (DAPI) and examined by fluorescence or differential interference contrast (DIC) microscopy.