Figure 4.

(A) Structure of the dst1 gene. The thick lines, open box, and solid box indicate the regions transcribed, a deduced ORF, and the intron, respectively. Two dst1 transcripts with distinct poly(A) binding sites were identified by 3′-RACE experiments. Vertical arrows indicate the 3′-ends of the dst1 transcripts. The primers for 5′- and 3′-RACE experiments, for the Northern probe, and for length polymorphism are indicated by horizontal half-arrows. (B) Schematic alignment of domains in Dst1 and WC-1. WC-1 is a blue-light receptor in N. crassa (Ballario et al. 1996). The vertical arrow beneath the Dst1 protein indicates the site of the dst1-1 mutation, which truncates AD in the C-terminal region.