Figure 6.

Expression of the wild-type dst1 gene. For each lane, 15 μg of total RNAs from the following were electrophoresed in a 1.0% agarose formaldehyde gel: vegetative mycelia cultured for 3 days (M), fruiting-body primordia of ∼2 mm in height (PR), young fruiting bodies on the day when fruiting-body maturation occurs (YP), pilei from young fruiting bodies (PI), stipe from young fruiting bodies (ST), and dark stipes (DST). After the ribosomal RNA was visualized under UV, the gel was blotted and hybridized. The probe was the 2640-bp PCR product including almost the entire ORF of dst1 (see Figure 4A). After hybridization, the blot was exposed to X-ray film. Similar results were reproducibly obtained in three independent experiments.