TABLE 5.
Mitotic and meiotic stability and dominance of Su[URE3]S and Su[URE3]I
| Mitotic stability (%)
|
[URE3] diploid (%)
|
Meiotic stability (%)
|
|||||
|---|---|---|---|---|---|---|---|
| Strain | 4∷0 | 3∷1 | 2∷2 | 1∷3 | 0∷4 | ||
| Su[URE3]S | 20 | 30 | 32 | 29 | 29 | 7 | 3 |
| Su[URE3]I | 92 | 90 | 90 | 7 | 3 | 0 | 0 |
Mitotic stability was determined by counting the ratio of [Ade+] clones after 20 generations in YPDA rich medium (2000 cells were counted for each strain). Dominance was determined by monitoring [Ade+] diploids obtained after crosses between wild-type and [URE3] strains (30 diploids were counted for each strain). After sporulation and dissection of the resulting [Ade+] diploids, meiotic stability was determined by analyzing the [URE3] segregation ([URE3]∷[ure0]) (120 tetrads were dissected for each strain).