TABLE 1.
Origin and resistance phenotype of individuals in this study
| Population (individual identifier)a | Locality | Pst + empty vectorb | Pst + AvrPto | Pto allelec | Trans. assaye |
|---|---|---|---|---|---|
| L. pimpinellifolium | |||||
| LA114 | Pacasmayo, Peru | S | S | pim114ψ | |
| LA373 | Culebras, Peru | S | R | pimPto2 | |
| LA400 | Haciendo Buenos Aires, Peru | S | R | pimPto | HR |
| LA411 | Pichilingue, Ecuador | S | R | pimPto2 | |
| L. parviflorum | |||||
| LA247 | Chavinillo, Peru | S | S | NDd | |
| LA1322 | Limatambo, Peru | R | R | parv80 | HR |
| LA2200 | Choipiaco, Peru | R | R | parv94 | HR |
| LA2133 | Ona, Ecuador | R | R | ND | |
| L. chmielewskii | |||||
| LA1306 | Tambo, Peru | R | R | chm106 | HR |
| LA2695 | Chihuanpampa, Peru | R | R | chm115 | HR |
| LA3653 | Matara, Peru | S | S | chmPto | HR |
| L. hirsutum | |||||
| LA 407 (c1) | Mirador, Ecuador | S | R | hir540 | |
| LA 407 (c2) | Mirador, Ecuador | S | R | ||
| LA1775 (7216) | Rio Casma, Peru | R | R | ND | |
| LA1775 (7219) | Rio Casma, Peru | hir46 | No HR | ||
| LA1777 (7220) | Rio Casma, Peru | R | R | ND | |
| PI 129157 (c1) | Banos, Ecuador | R | R | hir132 | HR |
| PI 129157 (c2) | Banos, Ecuador | S | R | ||
| PI 134417 (c1) | Ferrocarril a la Costa, Ecuador | R | R | hir183 | HR |
| PI 134417 (c2) | Ferrocarril a la Costa, Ecuador | S | R | ||
| PI 134418 (c1) | Ferrocarril a la Costa, Ecuador | R | R | hir186 | HR |
| PI 134418 (c2) | Ferrocarril a la Costa, Ecuador | R | R | ||
| PI 251305 (c1) | Sibambe, Ecuador | R | R | hir137 | HR |
| PI 251305 (c2) | Sibambe, Ecuador | R | R | ||
| L. pennellii | |||||
| LA716 | Atico, Peru | S | S | ND | |
| LA1912 | Cerro Locari, Peru | S | S | ND | |
| LA3791 | Caraveli, Peru | S | S | ND | |
| L. chilense | |||||
| LA460 (c1) | Palca, Peru | R | R | ||
| LA460 (c2) | Palca, Peru | R | R | ||
| LA2884 (7177) | Ayaviri, Chile | chi487 | No HR | ||
| LA2884 (7179) | Ayaviri, Chile | chi489 | HR | ||
| LA2884 (7180) | Ayaviri, Chile | chi493 | |||
| LA2884 (7183) | Ayaviri, Chile | chi497 | |||
| LA2884 (7184) | Ayaviri, Chile | chi502 | |||
| LA2884 (7185) | Ayaviri, Chile | S | R | chi580 | HR |
| LA2750 (1) | Mina La Despreciada, Chile | S | S | chi558 | No HR |
| LA2750 (2) | Mina La Despreciada, Chile | S | S | chi591 | |
| LA3355 (7186) | Cacique de Ara, Peru | R | R | chi151 chi551 | HR |
| LA3355 (7189) | Cacique de Ara, Peru | S | R | chi151 chi620 | HR |
| LA3355 (7190) | Cacique de Ara, Peru | R | R | chi566 chi565 | |
| L. peruvianum | |||||
| LA2744 (1) | Sobraya, Chile | S | S | peru554 | HR |
| LA2744 (2) | Sobraya, Chile | S | S | peru554 | HR |
| LA2744 (7232) | Sobraya, Chile | peru505ψ peru602ψ | |||
| LA2744 (7233) | Sobraya, Chile | peru603 | |||
| LA2744 (7234) | Sobraya, Chile | S | S | peru515 peru605 | |
| LA2744 (7235) | Sobraya, Chile | peru517 peru518 | HR | ||
| LA2744 (7236) | Sobraya, Chile | peru608 peru521 | |||
| LA3636 (7252) | Coayllo, Peru | S | R | peru592 | No HR |
| LA3636 (7258) | Coayllo, Peru | S | R | peru570 | No HR |
| LA3636 (7259) | Coayllo, Peru | S | R | peru594 | |
| LA3636 (7260) | Coayllo, Peru | S | R | peru567 | HR |
| PI126444 (1) | Rio Canta, Peru | S | S | peru245 peru246 | |
| PI126444 (2) | Rio Canta, Peru | S | R | ||
| PI128654 (1) | Azapa Valley, Chile | S | R | peru234 peru236 | |
| PI128654 (2) | Azapa Valley, Chile | S | S | ||
| PI128659 (1) | Tacna, Peru | S | S | peru238 peru239 | |
| PI128659 (2) | Tacna, Peru | S | R | peru243 | |
| LA2151 (1) | Morochupa, Peru | R | R | ||
| LA2151 (2) | Morochupa, Peru | R | R | ||
| LA3218 (1) | Arequipa, Peru | R | R | ||
| LA3666 (1) | Ica, Peru | R | S | ||
a
Identifier of specific individuals of these populations.
b
Plants resistant (R) or susceptible (S) when inoculated with isogenic strains of Pst expressing empty vector or avrPto based on pathogen growth.
c
Name of Pto allele isolated from this individual.
d
Pto allele not detected (ND) even though multiple independent PCR reactions were attempted. In all individuals, alleles of other paralogs were amplified and sequenced, indicating that the gene family does exist in these individuals.
e
Results of transient expression of these Pto alleles in N. benthamiana (HR, hypersensitive response).