TABLE 2.

Oligonucleotides used in this study

Oligo	Sequence	Position/purpose
OJA22	atc gtc agc cgt tga tga gc	606 bp upstream of the gpgA ATG
OJA27	CCGctc gag agg tcg cga gcg ga	5′ flanking region of the gpgA ORF for gpgA deletion with XhoI tail
OJA23	gct cgt aga tac tag tgt ag	573 bp downstream of the gpgA stop
OJA28	CCGctc gag gct gct gta cga tca t	3′ flanking region of gpgA ORF for gpgA deletion with XhoI tail
OJA24	ccg gaa gcg caa tca atc	sequencing the gpgA ORF, RT-PCR product and gpgA deletion confirmation
OJA25	tag cgg ccg ggg gta tca	Sequencing the gpgA ORF, RT-PCR product, and gpgA deletion confirmation
OJA26	ctg caa gac gct tga act g	3′ of the gpgA ORF
OJA29	cct gca tta gat gct tta	5′ of the gpgA ORF
OKH43	gcc aca ttc acg ata gcc	5′ of the fadA ORF
OKH44	atg aaa gtc tca acg cca	3′ of the fadA ORF
OKH01	gaa aac cac caa cag tgc	5′ of the sfaD ORF
OKH02	ttc ttt cca gat gat ccg	3′ of the sfaD ORF
OJA133	gag att cga gcc tgt gc	5′ of the flbA ORF
OJA134	ctg tca tga acg ttg tg	3′ of the flbA ORF
OYG4	tca caa cct cct ctc tca g	5′ of the fluG ORF
OYG5	ttg aac ggc tca cgg tac ta	3′ of the fluG ORF