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The truncation of Srb7 causes reduced association of the tail module with the middle (Med7) and head (Med8) domains of mediator. SRB7 (+) and srb7-1 (−) cells carrying either a Flag-tagged Med7 (left and right, lanes 1 and 2) or a Flag-tagged Med8 (left, lanes 3 and 4) were grown at 30°, harvested, and immunoprecipitated with Anti-Flag M2 monoclonal antibodies. These precipitates were then immunoblotted with Sin4, Gal11, Pgd1, or Flag antibodies as indicated.
