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. 2005 Oct;171(2):503–515. doi: 10.1534/genetics.105.042333

Figure 1.

Figure 1.

Figure 1.

MCM10 is required for proper telomeric and HMR silencing. (A) Telomeric silencing assays with mcm10 mutants. Strains ILY171, ILY180, ILY270, and GCY23, transformed with either pRS315 (top three rows and bottom row) or pRS315MCM10 (rows labeled “+MCM10”) are shown. Tenfold serial dilutions of overnight cultures were plated on CM-LEU media with or without the indicated concentration of 5-FOA. Plates were grown for 2 days at 30°. (B) Silencing assays of mcm10 mutants using the ADE2 reporter gene. Cultures were grown overnight in liquid selective media, diluted, and plated on CM-URA plates at ∼300 cells/plate. Plates were grown for 3 days at 30° and then incubated at 4° for 3 days before colonies were scored for color. Strains ILY171, ILY180, and ILY270 were used for the left and ILY163, ILY165, and ILY167 for the right. All strains were transformed with pRS315 (−) or pRS315MCM10 (+). All data points are the averages of at least two trials. Error bars that appear to be missing are too small to be displayed.