Figure 3.
Loci in T. cruzi where DTU IIa/IIc alleles are more similar to DTU IIb. (A) Unrooted neighbor-joining trees of HSP60 and 1F8 intergenic sequences reveal four DNA sequence classes. Superscript b or c represent DTU IIb or DTU IIc sequence types, respectively. **, sequence from CL Brener genomic read TCKCA96TR. (B) Agarose gel electrophoresis of PCR-RFLP products from the HSP60 locus of 25 strains. The DNA was stained with ethidium bromide. Size markers (M) are the BRL 1-kb ladders. • and ○ represent 0.5-kb and 0.1-kb bands, respectively. A SNP at position 125 creates the EcoRV polymorphism in the HSP60 locus (Table 2).