TABLE 1.
Anchor loci and primers
| Gene name | Abbreviation | Primer sequences | Intron(s)? |
|---|---|---|---|
| Dopa-decarboxylase | Ddc | Ddc-fo: CAGAGGGTCAAGGAACAGCAC | Yes |
| Ddc-ri: CGAAAGCGCAAGACGATGTAG | |||
| Decapentaplegic | Dpp | Dpp-f34: AGAGAACGTGGCGAGACACTG | No |
| Dpp-r327: GAGGAAAGTTGCGTAGGAACG | |||
| Patched | Ptc | Ptc-F(7): CTCCGAAGAAGGTCTGCCGCAAG | Yes |
| Ptc-R(364): AATTCGTGCTCGTCGTATTTTC | |||
| Cubitus interruptus | Ci | Ci-fi: ATGCGGAGACATACTGGTGAA | Yes |
| Ci-ro: TGTATCTTTTAGTGCAACCCG | |||
| Apterous | Ap | Ap-f35: TGAATCCTGAATACCTGGAGA | No |
| Ap-r286: CTTTTCCGCCATTTTGTTCTC | |||
| Vermilion | V | Ve-220f: CGCTCGACCTTATGGATTTC | Yes |
| Ve-465r: GAGTGTTAAGTCCCGGCGTA | |||
| Ribosomal protein L3 | RpL3 | RpL3-F59: CCGTCATCGTGGTAAAGTGA | Yes |
| RpL3-R537: TCTCCATGATATGGGCCTTC | |||
| Ribosomal protein L5 | RpL5 | RpL5-F23: CACCCAAGTACCGTTTGATTG | Yes |
| RpL5-R441: CATATCCTGGGAATCTCTTGATG | |||
| Ribosomal protein L10a | RpL10 | RpL10-F68: AATGCGGTCCTTCAATCATC | Yes |
| RpL10-R665: GTCCCATGGTGGACTTCATA | |||
| Ribosomal protein L11 | RpL11 | RpL11-F138: CTGTGTCGGTGAATCTGGTG | Yes |
| RpL11-R571: CTGTTGGAACCACTTCATGG | |||
| Ribosomal protein L19 | RpL19 | RpL19-F131: CCCGACAGAACATCCGTAAG | Yes |
| RpL19-R529: CGGGCTTCCTTCACTCTGT | |||
| Ribosomal protein P0 | RpP0 | RpP0-F142: ACCCAAAATGTTTCATCGTG | Yes |
| RpP0-R565: TCACCAGGCTTCAAGATGTG | |||
| Ribosomal protein S5 | RpS5 | RpS5-F31: GGTTGAGGAAAACTGGAACG | No |
| RpS5-R535: CAACAGCCAGATAGCCTGGT | |||
| Ribosomal protein S8 | RpS8 | RpS8-F56: GCCCATTCGTAAGAAGAGGAA | Yes |
| RpS8-R624: CTTTGCCCTCTTGGATTTGA | |||
| Ribosomal protein S9 | RpS9 | RpS9-F12: GCCATCATGGTGAACAACAG | Yes |
| RpS9-R601: TCTTCCTCCTCATCATTGGT | |||
| Elongation factor 1α | Ef1a | EF1H-F: GAGAAGGAAGCCCAGGAAAT | No |
| EF1H-R: CCTTGACRGACACGTTCTTT | |||
| Long-wavelength opsin | OPS1 | 5′-RACER: TGGTTACAATAGGGCTGA | Yes |
| RLWFD4: ACAAAAATCAATGGGAGAGTA | |||
| Triose-phosphate isomerase | Tpi | Tpi-1: GGTCACTCTGAAAGGAGAACCATCTT | Yes |
| Tpi-2: CACAACATTTGCCCAGTTGTTGCCAA | |||
| Mannose-phosphate isomerase | Mpi | Mpi-F: ATTCAAGCTCATCCAACTAAGG | Yes |
| Mpi-R: TTATGAAGTTGTTCTGCATGGT |
Full gene names, abbreviations, and primer sequences are shown. Primers are shown 5′–3′ with the forward primer listed first. “Introns (?)” indicates whether the fragment amplified contains one or more introns. OPS1, Tpi, Mpi, ci, dpp, and Ddc primers were published previously (Hsu et al. 2001; Beltrán et al. 2002; Tobler et al. 2004).