TABLE 2.
Microsatellite loci and primer sequences
| Locus | Primer sequence | Cross type | Allele sizes (bp) |
|---|---|---|---|
| Hm01 | CGCGGTAGAAATAGCACAAG | AB × C0 | 159, 168, 176, null |
| CGAGAAGCCCTACAAGTGTG | |||
| Hm02 | TATTTGCACGATGGAAACCC | AB × A0 | 170, 178, null |
| GCGAGGTGGAGACAAAAGAC | |||
| Hm03 | GACGTCACAGCGGGGAAC | AB × CD | 298, 324, 376, 444 |
| AGAGGGGAACGGAGTGTCAT | |||
| Hm04 | CCTGGCTTATCTACGACGACA | AB × A | 378, 392 |
| ATGCAGCTTACTCGCTGGTT | |||
| Hm05 | GCGGTAAGGTAAAACCGTGA | A0 × B0 | 150, 161, null |
| CAGAAGAAAATGGTTGGATGG | |||
| Hm06 | AAATAGTGTGCGGCGGAATA | AB × AA | 219, 221 |
| TGGAGTAGAAATGCGGGTTTA | |||
| Hm07 | GCAGAGGGAACCTCGTGTTA | A0 × B0 | 264, 266, null |
| CGCAGTTTGTGCGAATTACA | |||
| Hm08 | AAAGCCTGAGTGCCGTATTG | AB × CD | 285, 293, 295, 299 |
| GCAATGTCAGCATCGAATGT | |||
| Hm09 | m13-CAACTGCAATGACCCATCAC | A0 × B0 | 200, 218, null |
| AATGTCGTGCTCCCATGAAG | |||
| Hm10 | m13-GGCCGCTTTGTAAGAATGTC | AB × CD | 220, 242, 246, 268 |
| TGTGTAAATGAAATCCATAATTGGTC | |||
| Hm11 | m13-TTCTGGTGTCTAGCGGTTATG | A0 × B0 | 404, 416, null |
| AATAGCGACCATGCTGAGAG | |||
| Hm12 | m13-TGTCTTATCATTGGCGTTGC | AB × CD | 255, 263, 264, 281 |
| CAACCGTCGTTCCAGACG | |||
| Hm13 | m13-TCACTAGTTTTCGGCTTATCG | A0 × B0 | 185, 195, null |
| AAGGCTAAATGATGCCTAAAG | |||
| Hm14 | m13-ATGCTGTAACCCGCATAGC | A0 × B0 | 157, 195, null |
| TGCATTTATGATGTAAAAGTTCG | |||
| Hm15 | m13-TTTCGCCACCATAATCTTTC | AB × 00 | 234, 241, null |
| CACATCGCAGGTATTCCATC | |||
| Hm16 | m13-CGGATAGACATTTGTTAAAGTGTG | AB × BC | 266, 282, 291 |
| ACGAGGATGCGGACTACG | |||
| Hm17 | CAACGAGATATTCCAGAGAGTGTT | A0 × B0 | 355, 375, null |
| TGGAACGTATAGAAAGCGCA | |||
| Hm18 | AATTACATATCGTTTCATTA | AB × C0 | 218, 224, 254, null |
| CTACGACAAGACCCTCCTGA | |||
| Hm19 | m13-CGCTAATTCAAAGGAAAGAGGA | AB × C0 | 170, 174, 198, null |
| AGTGCTGTCATGGCTAACGA | |||
| Hm20 | GGGATCGATGAAAAAGAGCA | AB × B0 | — |
| AGAGCCTTCATTTACCCCGT | |||
| Hm21 | GAACTCCAGAAGGTTACCCCA | AB × BB | — |
| GCCGGTCTTTGTCTATTGGA | |||
| Hel.05 | TGCTGTCCATACCCAACTCA | AB × BC | 280, 294, 330 |
| CGAACTCACAACCATCAGTCA | |||
| Hel.17 | GGCGCACAGACGAGACTAC | A0 × B0 | 201, 207, null |
| ACTGCCGCACGAATAATAAC |
Primers are shown 5′–3′ with the forward primer listed first. The m13 sequence used in amplifications with m13-tailed primers is 5′-CACGACGTTGTAAAACGAC-3′ (see text for details). Segregation patterns and allele sizes are also shown. Hm04 is sex linked. Null alleles are shown as 0 in the segregation pattern column. Hm20 and Hm21 were scored on agarose gels so exact allele sizes are not shown.