TABLE 5.
Examples of genes whose expression changed from 24 to 40 hr
| Fold change
|
Experimental group
|
In pupae
|
||||
|---|---|---|---|---|---|---|
| P-value | Twofold | Fivefold | Gene | Comment | ||
| −11.38 | 0.01 | 5 | Yes | No | scute (sc) | Transcription factor, bristle development |
| −7.65 | 0.01 | 5 | No | No | Domina (Dom) | Required for imaginal disc growth. |
| −5.35 | 0.00 | 5 | Yes | No | Twin of m4 (Tom) | Thoracic bristle phenotype |
| −3.91 | 0.04 | No | No | non-stop (not) | Ubiquitin hydrolase, eye planar polarity | |
| −3.58 | 0.04 | No | No | Daughters against dpp (Dad) | dpp pathway | |
| −3.55 | 0.01 | Yes | No | Cyclin B (CycB) | Cell division | |
| −2.90 | 0.02 | Yes | No | Ubiquitin C-terminal hydrolase (UchL3) | Ubiquitin hydrolase | |
| −2.63 | 0.02 | Yes | No | wingless (wg) | Secreted factor, wing growth and margin | |
| −2.42 | 0.01 | No | No | schnurri (shn) | Transcription factor, dpp signaling | |
| −2.27 | 0.02 | No | No | Apc2 | Wnt signaling | |
| −2.06 | 0.04 | No | No | brinker (brk) | dpp signal transduction, wing growth | |
| 2.01 | 0.05 | No | No | puckered (puc) | JUN kinase phosphatase | |
| 2.21 | 0.01 | No | No | singed (sn) | Fascin, actin bundling, hair and bristle | |
| 2.57 | 0.05 | Yes | No | ebony (e) | Mutations result in dark body | |
| 2.59 | 0.04 | No | No | Moesin (Moe) | Disc cell morphology, cytoskeleton | |
| 2.65 | 0.05 | No | No | Tyramine beta hydroxylase (Tbh) | Catecholamine metabolism | |
| 2.76 | 0.05 | Yes | No | Stubble (Sb) | Endopeptidase, short stout bristles | |
| 3.02 | 0.03 | No | No | krotzkopf verkehrt (kkv) | Blimp embryonic cuticle phenotype | |
| 3.13 | 0.01 | No | No | Dopa decarboxylase (Ddc) | Cuticle crosslinking and pigmentation | |
| 3.47 | 0.00 | No | No | Rala | Ras family GTPase, hairs and bristles | |
| 3.87 | 0.00 | Yes | No | CG11546 | Drosophila kermit, planar polarity | |
| 4.18 | 0.01 | 2 | Yes | No | karst (kst) | Heavy β-spectrin, wing |
| 5.58 | 0.00 | 2 | No | No | ken and barbie (ken) | Transcription factor, loss of genitalia |
| 5.67 | 0.04 | 2 | No | No | knickkopf (knk) | Embryonic blimp phenotype |
| 6.02 | 0.00 | 3 | Yes | No | cheerio (cher) | Filamen protein, actin binding, female sterile |
| 6.46 | 0.00 | 2 | Yes | No | pawn (pwn) | Mutations affect hairs and bristles |
| 7.47 | 0.01 | 3 | Yes | No | Dopamine N-acetyltransferase (Dat) | Catecholamine metabolism |
| 8.11 | 0.04 | 3 | No | No | quail (qua) | Gelsolin/villin, actin bundling, female sterile |
| 11.84 | 0.01 | 1 | Yes | Yes | CG13209 | kojak gene (N. Ren and P. N. Adler, unpublished data) |
| 34.51 | 0.00 | 2 | Yes | Yes | yellow-d2 | Related to yellow |
| 37.22 | 0.00 | 2 | Yes | No | Buffy | Regulator of apoptosis |
| 39.77 | 0.03 | 2 | Yes | No | CG12045 | Cuticle protein |
| 42.53 | 0.03 | 2 | Yes | Yes | CG1869 | Chitinase |
| 58.05 | 0.02 | 3 | Yes | Yes | miniature (m) | Related to dy, small wing phenotype |
| 59.33 | 0.01 | 7 | No | No | Hormone receptor-like in 46 (Hr46) | Nuclear receptor; wing, bristle phenotypes |
| 99.26 | 0.00 | 2 | Yes | Yes | Drip | Aquaporin-like |
| 329.89 | 0.05 | 2 | No | No | dy (dusky) | Small dark wing, related to m |
| 398.96 | 0.00 | 2 | Yes | Yes | CG15013 | Related to dy |
| 467.26 | 0.05 | 2 | Yes | Yes | Myosin heavy chain (Mhc) | Muscle protein |
| 4589.75 | 0.02 | 2 | Yes | Yes | osi1 | Member of osirus family |