TABLE 3.
Loss of Pl′ paramutagenicity seen in Pl-Rh/Pl-Rh × Pl′/Pl′; rmr6-1/rmr6-1 testcrosses
| Staminate parenta
|
Pistillate tester
|
No. of testcrosses
|
No. of progeny with specific anther color scores
|
||||||
|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7b | |||
| 98-273-17 (F2) | W23 | 2 | 0 | 8 | 3 | 1 | 4 | 2 | 20 (53) |
| 98-354 (F2) | W23 | 2 | 2 | 17 | 10 | 4 | 1 | 1 | 9 (20) |
| 98-1339-4 (F3) | W23 | 1 | 1 | 12 | 6 | 1 | 0 | 0 | 6 (23) |
| 98-1339-8 (F3) | W23 | 1 | 2 | 6 | 4 | 0 | 0 | 0 | 12 (50) |
| 98-1339-12 (F3) | W23 | 1 | 0 | 6 | 8 | 0 | 0 | 0 | 1 (7) |
| 98-1340-1 (F3) | W23 | 1 | 0 | 2 | 4 | 0 | 0 | 3 | 15 (62) |
| 98-1342-9 (F3) | W23 | 1 | 0 | 5 | 4 | 0 | 0 | 0 | 14 (61) |
| 99-141-4 (F4) | W23 | 1 | 0 | 8 | 2 | 1 | 1 | 0 | 10 (45) |
| 99-142-24 (F4) | W23 | 1 | 0 | 0 | 1 | 1 | 2 | 0 | 11 (73) |
| 99-315-1 (F4) | W23 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 14 (100) |
| 97-740-7 (M2) | W23/CO159 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 6 (86) |
| 98-151-13 (F2) | W23/CO159 | 2 | 3 | 13 | 5 | 3 | 4 | 0 | 5 (15) |
| 98-354 (F2) | W23/CO159 | 4 | 19 | 34 | 10 | 9 | 5 | 0 | 9 (10) |
| 99-142-24 (F4) | W23/CO159 | 1 | 0 | 5 | 1 | 0 | 0 | 2 | 10 (56) |
| 99-217-7 (F4) | W23/CO159 | 2 | 8 | 17 | 0 | 1 | 0 | 1 | 0 (0) |
| 99-250-7 (F2) | W23/CO159 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 17 (85) |
| 99-250-24 (F2) | W23/CO159 | 2 | 7 | 11 | 1 | 4 | 0 | 0 | 10 (30) |
| Totals | 25 | 42 | 148 | 59 | 25 | 17 | 9 | 169 (36) | |
Pedigree origins of rmr6-1 homozygotes are indicated in parentheses: M2 is the family in which rmr6-1 homozygotes were first found, F2 indicates families in which Pl-Rh phenotypes were recovered following self-pollination of Rmr6/rmr6-1 plants with Pl′-like phenotypes, F3 and F4 indicate families generated by intercrossing heterozygous (Rmr6/rmr6-1) individuals with homozygous rmr6-1 F2 or F3 siblings, respectively.
The percentage of testcross progeny with ACS 7 is given in parentheses.