TABLE 5.
Testcross results measuring heritable loss of Pl′ paramutagenicity following transmission from rmr6-2/rmr6-2 plants
| Progeny structural genotype
|
No. of progeny with specific anther color scores
|
|||
|---|---|---|---|---|
| Testcrossa | 1–4 | 5–6 | 7 | |
| 02-490-8 × 02-470 | T/+ | 1 | 2 | 5 |
| 02-470 × 02-490-8 | +/T | 0 | 0 | 6 |
| 02-313 × 02-490-8 | +/T | 0 | 0 | 9 |
| 02-234 × 02-490-3 | +/T | 0 | 0 | 11 |
| 02-470 × 02-490-10 | +/T | 2 | 0 | 8 |
| 02-313 × 02-490-13 | +/T | 1 | 0 | 7 |
| 02-490-8 × 02-470 | +/+ | 0 | 0 | 10 |
| 02-470 × 02-490-8 | +/+ | 1 | 0 | 4 |
| 02-313 × 02-490-8 | +/+ | 0 | 0 | 9 |
| 02-234 × 02-490-3 | +/+ | 0 | 0 | 5 |
| 02-470 × 02-490-10 | +/+ | 1 | 0 | 7 |
| 02-313 × 02-490-13 | +/+ | 0 | 0 | 8 |
| Totals | T/+ | 1 | 2 | 5 |
| +/T | 3 | 0 | 41 | |
| +/+ | 2 | 0 | 43 | |
Experimental design is similar to that described in Figure 3 for rmr6-1.
Reciprocal crosses with a W23 Pl-Rh/Pl-Rh stock (02-234, 02-313, and 02-470) are indicated in which the pistillate parent is listed first. Italic numbers represent individual F1 plants from family 02-490 (materials and methods) having an ACS 7 anther phenotype (rmr6-2/rmr6-2) and ∼50% aborted pollen grains (+/T).